Hippocampus Window Implant:

See the original publication here

JoVE.JPG

The HC implant kit contains: i) assembled HC implant; ii) headplate; iii) infusion cannula.

General introduction to the hippocampus window.

Here, cannulas are soldered together.

Injection Cannula composed of

dummy cannula & infusion cannula

Microscope Cannula

Assembled HC implant

Headplate1.jpg

Headplate

Infusion cannula

5 mm

5 mm

After implanting the window onto the dorsal CA1 region on mice, viruses could be injected via the infusion cannula directly under the window.

Calcium Imaging

Voltage Imaging

VivoImaging.png

In Vivo Imaging

Combined with wide-field imaging, calcium and voltage imaging could be performed on the awake mice. 

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